lipofectamine tm 2000 kit (Thermo Fisher)
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lipofectamine tm 2000 kit
Lipofectamine Tm 2000 Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lipofectamine+tm+2000+kit/lipofectamine+3000+kit/pmc11717746-84-23-28
Average 90 stars, based on 1 article reviews
Lipofectamine Tm 2000 Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lipofectamine+tm+2000+kit/lipofectamine+3000+kit/pmc11717746-84-23-28
Average 90 stars, based on 1 article reviews
lipofectamine tm 2000 kit - by Bioz Stars,
2026-09
90/100 stars
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Transfection:Article Title: Curcumol enhances cisplatin sensitivity of gastric cancer: involvement of microRNA-7 and the nuclear factor-kappa B/snail family transcriptional repressor 1 axis Article Snippet: The miR-7 inhibitor, miR-7 control, small interfering (si) RNA of RELA (si-RELA) and the negative control (NC, RELA-NC), SNAIL and NC (RiboBio Co., Ltd., Guangzhou, Guangdong, China) were inserted into the pcDNA 3.1 vectors. .. The vectors were transfected into cells using a Article Title: E74-like ETS transcription factor 1 promotes the progression of pancreatic cancer by regulating doublecortin-like kinase 1/Janus kinase/signal transducer and activator of transcription pathway Article Snippet: Cell transfection Empty plasmid (negative control, NC), ELF1 overexpression plasmid (ELF1), DCLK1 overexpression plasmid (DCLK1), small interfering RNA (siRNA) targeting ELF1 (si-ELF1) (si-ELF1-1: sense, 5’-CACUUCAAAUAGGAAUCAAC-3’; anti-sense, 5’-GUUGAUUCCUAUUUGAAGUG-3’; si-ELF1-2: sense, 5’-UCCGACCGAGUCGUCCAUGUA-3’; anti-sense, 5’-UACAUGGACGACUCGGUCGGA-3’), siRNA targeting DCLK1 (si-DCLK1; sense, 5’-GAUCGAUACUUCAAAGGGA-3’; anti-sense, 5’-UCCCUUUGAAGUAUCGAUC-3’), and negative control (si-NC; sense, 5’-UUCUCCGAACGUGUCACGUTT-3’; anti-sense, 5’-ACGUGACACGUUCGGAGAATT-3’) were purchased from GenePharma (Shanghai, China). .. When the cells reached 60-80% confluence, the above plasmids or siRNAs were transfected into Capan-2 and PANC-1 cells with Article Title: Exosomal microRNA-503-3p derived from macrophages represses glycolysis and promotes mitochondrial oxidative phosphorylation in breast cancer cells by elevating DACT2 Article Snippet: .. P4-P6 macrophages at 80% confluence were transfected with miR-503-3p inhibitor, miR-503-3p mimic, or the negative control (NC) (GenePharma Co., Ltd., Shanghai, China) based on Article Title: miR-212-5p inhibits nasopharyngeal carcinoma progression by targeting METTL3 Article Snippet: When cells reached 80–90% confluence, cells were trypsinized with 0.25% trypsin (Roche, Basel, Swizerland). .. Then, the cells were inoculated into six-well plates at the density of 1 × 10 5 cells/ml and incubated for 24 h at 37°C in 5% CO 2 . miR-212-5p mimic (miR-212-5p: 3′-UCAUUCGUCAGAUCUCGGUUCCA-5′), mimics control (miR-NC: 5′-UUCUCCGAACGUGUCACGUTT-3′), miR-212-5p inhibitor (miR-212-5p-in: 3′-UGGAACCGAGAUCUGACGAAUGA-5′), inhibitors control (miR-in: 5′-UUGUACUACACAAAAGUACUG-3′), empty vector (NC), METTL3 overexpression plasmid (METTL3), small interfering RNA (siRNA) targeting METTL3 (si-METTL3), and siRNA normal control (si-NC) from GenePharma (Shanghai, China) were subsequently transfected into 5-8F and 6-10B cells with Article Title: Pterostilbene suppresses the growth of esophageal squamous cell carcinoma by inhibiting glycolysis and PKM2/STAT3/c-MYC signaling pathway. Article Snippet: Pterostilbene (PTS) is a dietary phytochemical that has shown antitumor activity in many types of cancer, but the molecular mechanism remains unclear.. It has also not been adequately studied on PTS against esophageal squamous cell carcinoma (ESCC).. Thus, this study investigated the effect of PTS on ESCC in vitro and in vivo and explored the underlying molecular mechanism. Article Title: MUC5B regulates alterations in the immune microenvironment in nasopharyngeal carcinoma via the Wnt/β-catenin signaling pathway Article Snippet: .. When the cell fusion degree reached 60–80%, the MUC5B abnormal expression vectors were transfected into the cells as per the instructions of the Negative Control:Article Title: Exosomal microRNA-503-3p derived from macrophages represses glycolysis and promotes mitochondrial oxidative phosphorylation in breast cancer cells by elevating DACT2 Article Snippet: .. P4-P6 macrophages at 80% confluence were transfected with miR-503-3p inhibitor, miR-503-3p mimic, or the negative control (NC) (GenePharma Co., Ltd., Shanghai, China) based on Article Title: Pterostilbene suppresses the growth of esophageal squamous cell carcinoma by inhibiting glycolysis and PKM2/STAT3/c-MYC signaling pathway. Article Snippet: Pterostilbene (PTS) is a dietary phytochemical that has shown antitumor activity in many types of cancer, but the molecular mechanism remains unclear.. It has also not been adequately studied on PTS against esophageal squamous cell carcinoma (ESCC).. Thus, this study investigated the effect of PTS on ESCC in vitro and in vivo and explored the underlying molecular mechanism. Article Title: Study on the Mechanism of miR-34a Affecting the Proliferation, Migration, and Invasion of Human Keloid Fibroblasts by Regulating the Expression of SATB1 Article Snippet: .. Fetal bovine serum (FBS) and DMEM medium were purchased from Gibco, US; MTT was purchased from Amresco, US; Incubation:Article Title: miR-212-5p inhibits nasopharyngeal carcinoma progression by targeting METTL3 Article Snippet: When cells reached 80–90% confluence, cells were trypsinized with 0.25% trypsin (Roche, Basel, Swizerland). .. Then, the cells were inoculated into six-well plates at the density of 1 × 10 5 cells/ml and incubated for 24 h at 37°C in 5% CO 2 . miR-212-5p mimic (miR-212-5p: 3′-UCAUUCGUCAGAUCUCGGUUCCA-5′), mimics control (miR-NC: 5′-UUCUCCGAACGUGUCACGUTT-3′), miR-212-5p inhibitor (miR-212-5p-in: 3′-UGGAACCGAGAUCUGACGAAUGA-5′), inhibitors control (miR-in: 5′-UUGUACUACACAAAAGUACUG-3′), empty vector (NC), METTL3 overexpression plasmid (METTL3), small interfering RNA (siRNA) targeting METTL3 (si-METTL3), and siRNA normal control (si-NC) from GenePharma (Shanghai, China) were subsequently transfected into 5-8F and 6-10B cells with Control:Article Title: miR-212-5p inhibits nasopharyngeal carcinoma progression by targeting METTL3 Article Snippet: When cells reached 80–90% confluence, cells were trypsinized with 0.25% trypsin (Roche, Basel, Swizerland). .. Then, the cells were inoculated into six-well plates at the density of 1 × 10 5 cells/ml and incubated for 24 h at 37°C in 5% CO 2 . miR-212-5p mimic (miR-212-5p: 3′-UCAUUCGUCAGAUCUCGGUUCCA-5′), mimics control (miR-NC: 5′-UUCUCCGAACGUGUCACGUTT-3′), miR-212-5p inhibitor (miR-212-5p-in: 3′-UGGAACCGAGAUCUGACGAAUGA-5′), inhibitors control (miR-in: 5′-UUGUACUACACAAAAGUACUG-3′), empty vector (NC), METTL3 overexpression plasmid (METTL3), small interfering RNA (siRNA) targeting METTL3 (si-METTL3), and siRNA normal control (si-NC) from GenePharma (Shanghai, China) were subsequently transfected into 5-8F and 6-10B cells with Over Expression:Article Title: miR-212-5p inhibits nasopharyngeal carcinoma progression by targeting METTL3 Article Snippet: When cells reached 80–90% confluence, cells were trypsinized with 0.25% trypsin (Roche, Basel, Swizerland). .. Then, the cells were inoculated into six-well plates at the density of 1 × 10 5 cells/ml and incubated for 24 h at 37°C in 5% CO 2 . miR-212-5p mimic (miR-212-5p: 3′-UCAUUCGUCAGAUCUCGGUUCCA-5′), mimics control (miR-NC: 5′-UUCUCCGAACGUGUCACGUTT-3′), miR-212-5p inhibitor (miR-212-5p-in: 3′-UGGAACCGAGAUCUGACGAAUGA-5′), inhibitors control (miR-in: 5′-UUGUACUACACAAAAGUACUG-3′), empty vector (NC), METTL3 overexpression plasmid (METTL3), small interfering RNA (siRNA) targeting METTL3 (si-METTL3), and siRNA normal control (si-NC) from GenePharma (Shanghai, China) were subsequently transfected into 5-8F and 6-10B cells with Plasmid Preparation:Article Title: miR-212-5p inhibits nasopharyngeal carcinoma progression by targeting METTL3 Article Snippet: When cells reached 80–90% confluence, cells were trypsinized with 0.25% trypsin (Roche, Basel, Swizerland). .. Then, the cells were inoculated into six-well plates at the density of 1 × 10 5 cells/ml and incubated for 24 h at 37°C in 5% CO 2 . miR-212-5p mimic (miR-212-5p: 3′-UCAUUCGUCAGAUCUCGGUUCCA-5′), mimics control (miR-NC: 5′-UUCUCCGAACGUGUCACGUTT-3′), miR-212-5p inhibitor (miR-212-5p-in: 3′-UGGAACCGAGAUCUGACGAAUGA-5′), inhibitors control (miR-in: 5′-UUGUACUACACAAAAGUACUG-3′), empty vector (NC), METTL3 overexpression plasmid (METTL3), small interfering RNA (siRNA) targeting METTL3 (si-METTL3), and siRNA normal control (si-NC) from GenePharma (Shanghai, China) were subsequently transfected into 5-8F and 6-10B cells with Article Title: Co-treatment With Everolimus, an mTOR-Specific Antagonist, or Downregulation of ELK1 Enhances the Sensitivity of Pancreatic Cancer Cells to Genistein Article Snippet: .. Then, the recombinant vector was co-transfected with oe-ELK3 into 293T cells using the Small Interfering RNA:Article Title: miR-212-5p inhibits nasopharyngeal carcinoma progression by targeting METTL3 Article Snippet: When cells reached 80–90% confluence, cells were trypsinized with 0.25% trypsin (Roche, Basel, Swizerland). .. Then, the cells were inoculated into six-well plates at the density of 1 × 10 5 cells/ml and incubated for 24 h at 37°C in 5% CO 2 . miR-212-5p mimic (miR-212-5p: 3′-UCAUUCGUCAGAUCUCGGUUCCA-5′), mimics control (miR-NC: 5′-UUCUCCGAACGUGUCACGUTT-3′), miR-212-5p inhibitor (miR-212-5p-in: 3′-UGGAACCGAGAUCUGACGAAUGA-5′), inhibitors control (miR-in: 5′-UUGUACUACACAAAAGUACUG-3′), empty vector (NC), METTL3 overexpression plasmid (METTL3), small interfering RNA (siRNA) targeting METTL3 (si-METTL3), and siRNA normal control (si-NC) from GenePharma (Shanghai, China) were subsequently transfected into 5-8F and 6-10B cells with Article Title: Study on the Mechanism of miR-34a Affecting the Proliferation, Migration, and Invasion of Human Keloid Fibroblasts by Regulating the Expression of SATB1 Article Snippet: .. Fetal bovine serum (FBS) and DMEM medium were purchased from Gibco, US; MTT was purchased from Amresco, US; Expressing:Article Title: MUC5B regulates alterations in the immune microenvironment in nasopharyngeal carcinoma via the Wnt/β-catenin signaling pathway Article Snippet: .. When the cell fusion degree reached 60–80%, the MUC5B abnormal expression vectors were transfected into the cells as per the instructions of the MTT Assay:Article Title: Study on the Mechanism of miR-34a Affecting the Proliferation, Migration, and Invasion of Human Keloid Fibroblasts by Regulating the Expression of SATB1 Article Snippet: .. Fetal bovine serum (FBS) and DMEM medium were purchased from Gibco, US; MTT was purchased from Amresco, US; Sequencing:Article Title: Study on the Mechanism of miR-34a Affecting the Proliferation, Migration, and Invasion of Human Keloid Fibroblasts by Regulating the Expression of SATB1 Article Snippet: .. Fetal bovine serum (FBS) and DMEM medium were purchased from Gibco, US; MTT was purchased from Amresco, US; Reverse Transcription:Article Title: Study on the Mechanism of miR-34a Affecting the Proliferation, Migration, and Invasion of Human Keloid Fibroblasts by Regulating the Expression of SATB1 Article Snippet: .. Fetal bovine serum (FBS) and DMEM medium were purchased from Gibco, US; MTT was purchased from Amresco, US; Polymerase Chain Reaction:Article Title: Study on the Mechanism of miR-34a Affecting the Proliferation, Migration, and Invasion of Human Keloid Fibroblasts by Regulating the Expression of SATB1 Article Snippet: .. Fetal bovine serum (FBS) and DMEM medium were purchased from Gibco, US; MTT was purchased from Amresco, US; Synthesized:Article Title: Study on the Mechanism of miR-34a Affecting the Proliferation, Migration, and Invasion of Human Keloid Fibroblasts by Regulating the Expression of SATB1 Article Snippet: .. Fetal bovine serum (FBS) and DMEM medium were purchased from Gibco, US; MTT was purchased from Amresco, US; Recombinant:Article Title: Co-treatment With Everolimus, an mTOR-Specific Antagonist, or Downregulation of ELK1 Enhances the Sensitivity of Pancreatic Cancer Cells to Genistein Article Snippet: .. Then, the recombinant vector was co-transfected with oe-ELK3 into 293T cells using the |